Enzyme lab Report Example | Topics and Well Written Essays - 500 words

Enzyme - Lab Report Example Conclusively, increase in temperature increases the activity of the enzyme up to the maximum level, and further increase leads to the denaturing of the enzyme. For any chemical reaction to proceed within a cell, activation energy is required to cause and complete this process. This is made possible by the use of enzymes. Enzymes essentially work within the cells and their ability determined as a result of their specificity brought about by the shapes from the amino acid sequences (Daniel and Danson 2740). The enzymes have active sites on their surfaces to allow the binding of a substrate through the help of coenzymes to form enzyme-substrate complex. The chemical reaction thus converts the substrate to a new product then released and the catalytic cycle proceeds. Many factors, for example, pH and temperature affects the way enzymes work by either increasing the rate or determining the type of product produced (). The report, therefore, analyses the effects of the enzyme peroxidase in metabolic reactions and determining its optimum temperature in the reactions. The effect of temperature was then determined on the activity of enzyme peroxidase through monitoring its effects under different temperatures of 4, 32 and 600C on the dye guaiacol when it turned brown. Temperature increased from 40 C to room temperature of 22.20 C resulting to the increase of the peroxidase activity. This explained that increase in temperature up to the optimum point led to the increase of the breakdown of guaiacol dye to color brown with intensity increasing up to this optimum point. Further increase in temperature to 60C denatured the enzyme and therefore the production of the brown color stopped and its intensity reduced. From the experiment therefore, the optimum temperature for peroxidase is at 22.20C from graph on figure 1. The temperature effect is reversible in the case whereby the peroxidase is exposed to temperatures that negatively affect